Issue 68, 2020

Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

Abstract

A terminal-closed linear gene with strong exonuclease resistance and serum stability was successfully constructed by polymerase chain reaction (PCR) with an α-L-threose nucleic acid (TNA) loop modified primer pair, which can be used as an efficient gene expression system in eukaryotic cells for gene delivery.

Graphical abstract: Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

Supplementary files

Article information

Article type
Communication
Submitted
23 Jun 2020
Accepted
18 Jul 2020
First published
21 Jul 2020

Chem. Commun., 2020,56, 9894-9897

Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

X. Lu, X. Wu, T. Wu, L. Han, J. Liu and B. Ding, Chem. Commun., 2020, 56, 9894 DOI: 10.1039/D0CC04356G

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