Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation

Xinyu Ling; Xiaoqin Gao; Liying Chang; Heqi Chen; Xiaomeng Shi; Tao Liu

doi:10.1039/D0CC01432J

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Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation†

Xinyu Ling,‡^a Xiaoqin Gao,‡^a Liying Chang,^a Heqi Chen,^a Xiaomeng Shi^a and Tao Liu

*^a

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* Corresponding authors

^a State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, Beijing 100191, China
E-mail: taoliupku@pku.edu.cn

Abstract

The CRISPR–Cas9 system enables facile and efficient genome engineering in living cells and organisms. We report a Cas9–RNA conjugation strategy to afford minimal crRNA containing only the guide sequence for the target gene, which may simplify and reduce the cost for large-scale and high-throughput crRNA synthesis and lead to new insights into the design of CRISPR family complexes for diverse purposes.

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Article information

DOI: https://doi.org/10.1039/D0CC01432J
Article type: Communication
Submitted: 24 Feb 2020
Accepted: 26 May 2020
First published: 02 Jun 2020

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Chem. Commun., 2020,56, 7515-7518

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Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation

X. Ling, X. Gao, L. Chang, H. Chen, X. Shi and T. Liu, Chem. Commun., 2020, 56, 7515 DOI: 10.1039/D0CC01432J

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Chemical Communications

Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation†

Abstract

Supplementary files

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Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation

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