Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale

Dmitriy V. Zhukov; Eugenia M. Khorosheva; Tahmineh Khazaei; Wenbin Du; David A. Selck; Alexander A. Shishkin; Rustem F. Ismagilov

doi:10.1039/C9LC00541B

Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale†

Dmitriy V. Zhukov,

^a Eugenia M. Khorosheva,^a Tahmineh Khazaei,^b Wenbin Du,

‡^c David A. Selck,^a Alexander A. Shishkin^b and Rustem F. Ismagilov

*^ab

Author affiliations

* Corresponding authors

^a Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd, Pasadena, CA, USA
E-mail: rustem.admin@caltech.edu

^b Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd, Pasadena, CA, USA

^c Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA

Abstract

We have developed a multistep microfluidic device that expands the current SlipChip capabilities by enabling multiple steps of droplet merging and multiplexing. Harnessing the interfacial energy between carrier and sample phases, this manually operated device accurately meters nanoliter volumes of reagents and transfers them into on-device reaction wells. Judiciously shaped microfeatures and surface-energy traps merge droplets in a parallel fashion. Wells can be tuned for different volumetric capacities and reagent types, including for pre-spotted reagents that allow for unique identification of original well contents even after their contents are pooled. We demonstrate the functionality of the multistep SlipChip by performing RNA transcript barcoding on-device for synthetic spiked-in standards and for biologically derived samples. This technology is a good candidate for a wide range of biological applications that require multiplexing of multistep reactions in nanoliter volumes, including single-cell analyses.

Supplementary files

Article information

DOI: https://doi.org/10.1039/C9LC00541B
Article type: Paper
Submitted: 06 Jun 2019
Accepted: 29 Jul 2019
First published: 23 Aug 2019
This article is Open Access

Download Citation

Lab Chip, 2019,19, 3200-3211

Permissions

Request permissions

Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale

D. V. Zhukov, E. M. Khorosheva, T. Khazaei, W. Du, D. A. Selck, A. A. Shishkin and R. F. Ismagilov, Lab Chip, 2019, 19, 3200 DOI: 10.1039/C9LC00541B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Lab on a Chip

Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale

Social activity

Search articles by author

Spotlight

Advertisements