A novel method to engineer proteases for selective enzyme inhibition

Lingling Li; Hai Shi; Anzhi Sheng; Yi Yang; Liu Shi; Chao Li; Genxi Li

doi:10.1039/C9CC08085F

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

A novel method to engineer proteases for selective enzyme inhibition†

Lingling Li,^a Hai Shi,^b Anzhi Sheng,^a Yi Yang,^b Liu Shi,^b Chao Li*^bc and Genxi Li

*^ab

Author affiliations

* Corresponding authors

^a Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, P. R. China
E-mail: genxili@nju.edu.cn

^b State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China
E-mail: lchao@hfut.edu.cn

^c School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, P. R. China

Abstract

In this work, we have proposed a new strategy to expand the function of a protein. By taking a protease as an example, it can be engineered to make up the shortcoming of natural proteases, and thus it can efficiently and selectively hydrolyze a desired protein even in a complex biological fluid.

Download options Please wait...

Supplementary files

Supplementary information PDF (321K)

Article information

DOI: https://doi.org/10.1039/C9CC08085F
Article type: Communication
Submitted: 15 Oct 2019
Accepted: 29 Oct 2019
First published: 30 Oct 2019

Download Citation

Chem. Commun., 2019,55, 14039-14042

Permissions

Request permissions

A novel method to engineer proteases for selective enzyme inhibition

L. Li, H. Shi, A. Sheng, Y. Yang, L. Shi, C. Li and G. Li, Chem. Commun., 2019, 55, 14039 DOI: 10.1039/C9CC08085F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications

A novel method to engineer proteases for selective enzyme inhibition†

Abstract

Supplementary files

Article information

Download Citation

Permissions

A novel method to engineer proteases for selective enzyme inhibition

Social activity

Search articles by author

Spotlight

Advertisements