Issue 78, 2019

Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

Abstract

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

Graphical abstract: Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

Supplementary files

Article information

Article type
Communication
Submitted
16 Jul 2019
Accepted
15 Aug 2019
First published
09 Sep 2019
This article is Open Access
Creative Commons BY license

Chem. Commun., 2019,55, 11671-11674

Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors

A. M. Thomas, S. Brolih, J. F. McGouran, A. H. El-Sagheer, D. Ptchelkine, M. Jones, N. Q. McDonald, P. J. McHugh and T. Brown, Chem. Commun., 2019, 55, 11671 DOI: 10.1039/C9CC05476F

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