Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors

Yasuaki Kimura; Zhaoma Shu; Mika Ito; Naoko Abe; Kosuke Nakamoto; Fumiaki Tomoike; Satoshi Shuto; Yoshihiro Ito; Hiroshi Abe

doi:10.1039/C9CC04872C

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Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors†

Yasuaki Kimura,

^a Zhaoma Shu,^a Mika Ito,^bc Naoko Abe,

^a Kosuke Nakamoto,

^a Fumiaki Tomoike,

^d Satoshi Shuto,

^b Yoshihiro Ito

^c and Hiroshi Abe

*^ace

Author affiliations

* Corresponding authors

^a Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan
E-mail: h-abe@chem.nagoya-u.ac.jp

^b Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan

^c Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1, Hirosawa, Wako-Shi, Saitama, Japan

^d Research Center for Materials Science, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8602, Japan

^e CREST, Japan Science and Technology Agency, 7, Gobancho, Chiyoda-ku, Tokyo, Japan

Abstract

We herein report a new approach for RNA interference, so-called “build-up RNAi” approach, where single-strand circular RNAs with a photocleavable unit or disulfide moiety were used as siRNA precursors. The advantages of using these circular RNA formats for RNAi were presented in aspects of immunogenicity and cellular uptake.

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Article information

DOI: https://doi.org/10.1039/C9CC04872C
Article type: Communication
Submitted: 25 Jun 2019
Accepted: 02 Dec 2019
First published: 02 Dec 2019

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Chem. Commun., 2020,56, 466-469

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Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors

Y. Kimura, Z. Shu, M. Ito, N. Abe, K. Nakamoto, F. Tomoike, S. Shuto, Y. Ito and H. Abe, Chem. Commun., 2020, 56, 466 DOI: 10.1039/C9CC04872C

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Chemical Communications

Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors†

Abstract

Supplementary files

Article information

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Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors

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