An endoplasmic reticulum-targetable fluorescent probe for highly selective detection of hydrogen sulfide

Hui Zhang; Jiali Chen; Haiqing Xiong; Yun Zhang; Wenqiang Chen; Jiarong Sheng; Xiangzhi Song

doi:10.1039/C8OB02998A

An endoplasmic reticulum-targetable fluorescent probe for highly selective detection of hydrogen sulfide†

Hui Zhang,^ab Jiali Chen,^b Haiqing Xiong,^a Yun Zhang,^a Wenqiang Chen,

*^b Jiarong Sheng^b and Xiangzhi Song

*^ac

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* Corresponding authors

^a College of Chemistry & Chemical Engineering, Central South University, Changsha, Hunan 410083, P. R. China
E-mail: xzsong@csu.edu.cn

^b College of Chemistry and Materials Science, Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning 530001, P. R. China
E-mail: chenwq@csu.edu.cn
Fax: +86 771 3908065

^c Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha, China

Abstract

Hydrogen sulfide (H₂S), a critical endogenous signaling molecule, is widely involved in many physiological processes. Endoplasmic reticulum, an important organelle with a sac-like structure, plays crucial roles in maintaining the normal function of cells. Accordingly, monitoring the H₂S levels in endoplasmic reticulum is of great importance. Herein, we have developed an endoplasmic reticulum-targetable fluorescent probe, ER-CN, for H₂S detection. ER-CN features excellent sensing properties, such as high sensitivity and selectivity. In addition, ER-CN exhibits low cytotoxicity and a fine endoplasmic reticulum targeting property (with a Pearson's colocalization coefficient of 0.95). Significantly, visualization of H₂S in the endoplasmic reticulum of living HeLa cells by using ER-CN was successfully realized.

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DOI: https://doi.org/10.1039/C8OB02998A
Article type: Paper
Submitted: 02 Dec 2018
Accepted: 16 Jan 2019
First published: 16 Jan 2019

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Org. Biomol. Chem., 2019,17, 1436-1441

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An endoplasmic reticulum-targetable fluorescent probe for highly selective detection of hydrogen sulfide

H. Zhang, J. Chen, H. Xiong, Y. Zhang, W. Chen, J. Sheng and X. Song, Org. Biomol. Chem., 2019, 17, 1436 DOI: 10.1039/C8OB02998A

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Organic & Biomolecular Chemistry

An endoplasmic reticulum-targetable fluorescent probe for highly selective detection of hydrogen sulfide†

Abstract

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An endoplasmic reticulum-targetable fluorescent probe for highly selective detection of hydrogen sulfide

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