Issue 12, 2018
From the journal:

Food & Function

Licochalcone A inhibits the invasive potential of human glioma cells by targeting the MEK/ERK and ADAM9 signaling pathways

Chien-Feng Huang,ab   Shun-Fa Yang,ac   Hui-Ling Chiou,d   Wen-Hung Hsu,e   Jen-Chieh Hsu,f   Chung-Jung Liu*eg  and  Yi-Hsien Hsieh ORCID logo *fh  

* Corresponding authors

a Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

b Department of Critical Care Medicine, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan

c Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan

d School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan

e Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
E-mail: pinkporkkimo@yahoo.com.tw

f Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan
E-mail: hyhsien@csmu.edu.tw

g Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan

h Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan

Abstract

Licochalcone A (LicA) has been reported to possess antitumor properties. However, its effect on human glioma cells remains unknown. In this study, we observed that LicA significantly suppressed the ADAM9 expression and the migration and invasion activities of human glioma cells (M059K, U-251 MG, and GBM8901) and exhibited no cell cytotoxicity. The human proteinase antibody array and immunoblot analysis indicated that the LicA treatment inhibited the expression of ADAM9 protein in human glioma cells. Recombinant human ADAM-9 (Rh-ADAM9) treatment significantly reversed the LicA-induced reduction in the ADAM9 level and the migration and invasion activities of human glioma cells. Additionally, the phosphorylation/activation of the mitogen-activated protein kinase kinase (MEK)–extracellularly responsive kinases (ERK) signaling pathway was significantly suppressed in LicA-treated human glioma cells. Cotreatment with LicA and PD98059 synergistically inhibited the ADAM9 expression, cell migration, and cell invasion, which suggested that the MEK–ERK signaling pathway was involved in the LicA-induced inhibition of the ADAM9 expression and the invasion activity of human glioma cells. These findings are the first evidence of LicA's anti-invasive properties against human glioma cells.

Graphical abstract: Licochalcone A inhibits the invasive potential of human glioma cells by targeting the MEK/ERK and ADAM9 signaling pathways

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Article information

DOI
https://doi.org/10.1039/C8FO01643G
Article type
Paper
Submitted
18 Aug 2018
Accepted
29 Oct 2018
First published
30 Oct 2018

Food Funct., 2018,9, 6196-6204

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Licochalcone A inhibits the invasive potential of human glioma cells by targeting the MEK/ERK and ADAM9 signaling pathways

C. Huang, S. Yang, H. Chiou, W. Hsu, J. Hsu, C. Liu and Y. Hsieh, Food Funct., 2018, 9, 6196 DOI: 10.1039/C8FO01643G

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