Self-assembled adhesive biomaterials formed by a genetically designed fusion protein

Pulakesh Aich; Jaeyeon An; Byeongseon Yang; Young Ho Ko; Junghyun Kim; James Murray; Hyung Joon Cha; Joon Ho Roh; Kyeng Min Park; Kimoon Kim

doi:10.1039/C8CC07475E

Self-assembled adhesive biomaterials formed by a genetically designed fusion protein†

Pulakesh Aich,‡^a Jaeyeon An,‡^b Byeongseon Yang,‡^c Young Ho Ko,^a Junghyun Kim,

^a James Murray,^a Hyung Joon Cha,

*^c Joon Ho Roh,*^a Kyeng Min Park

*^a and Kimoon Kim

*^ab

Author affiliations

* Corresponding authors

^a Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, Republic of Korea
E-mail: jhroh@ibs.re.kr, kmpark@ibs.re.kr, kkim@postech.ac.kr
Web: http://csc.ibs.re.kr

^b Department of Chemistry, Pohang University of Science and Technology, Pohang, Republic of Korea

^c Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea
E-mail: hjcha@postech.ac.kr
Web: http://magic.postech.ac.kr

Abstract

Here we report a recombinant protein (MS) obtained by genetic fusion of a mussel foot protein (Mfp3) motif into a silk spidroin (MaSp1). The MS not only self-assembled into a supramolecular fibre, as does the parent MaSp1, but also showed enhanced adhesiveness resulting from the DOPA-containing Mfp3 portion. The successful incorporation of the wet adhesiveness of Mfp3 into the well-structured assembly of MaSp1 may provide a new insight for the genetic design of underwater adhesive recombinant proteins by utilizing the structural features of a spidroin protein.

Chemical Communications

Self-assembled adhesive biomaterials formed by a genetically designed fusion protein†

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Self-assembled adhesive biomaterials formed by a genetically designed fusion protein

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