Issue 1, 2018

Structure characterization of a novel polysaccharide from Hericium erinaceus fruiting bodies and its immunomodulatory activities

Abstract

A novel polysaccharide fraction (HEP-S) was extracted and isolated from the fruiting bodies of Hericium erinaceus. Structural characterization revealed that HEP-S had an average molecular weight of 1.83 × 104 Da and consisted of rhamnose, fucose, mannose, glucose and galactose at a molar ratio of 1.47 : 0.93 : 1.36 : 8.68 : 4.08. Periodate oxidation–Smith degradation and NMR analysis showed that the main linkage types of HEP-S were composed of (1→)-α-D-Glc, (1→3,4)-α-D-Glc, (1→6)-α-D-Gal, (1→3,4)-β-D-Man, (1→3,6)-α-Rha and (1→2)-β-L-Fuc. The immunomodulatory assay indicated that HEP-S could significantly enhance the pinocytic and phagocytic capacity and promote the secretion of nitric oxide and pro-inflammatory cytokines by activating the corresponding mRNA and protein expression in RAW 264.7 cells involving a toll-like receptor 2 membrane receptor. Besides, HEP-S was also found to improve the adaptive immune function by enhancing T and B lymphocyte proliferation and increasing the interleukin-2, interleukin-4 and interferon-γ secretion in spleen lymphocytes. These results suggested that HEP-S could be used as a potential immunoregulatory agent in functional foods.

Graphical abstract: Structure characterization of a novel polysaccharide from Hericium erinaceus fruiting bodies and its immunomodulatory activities

Article information

Article type
Paper
Submitted
07 Sep 2017
Accepted
06 Nov 2017
First published
09 Nov 2017

Food Funct., 2018,9, 294-306

Structure characterization of a novel polysaccharide from Hericium erinaceus fruiting bodies and its immunomodulatory activities

F. Wu, C. Zhou, D. Zhou, S. Ou, X. Zhang and H. Huang, Food Funct., 2018, 9, 294 DOI: 10.1039/C7FO01389B

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