Issue 61, 2017
From the journal:

Chemical Communications

Single antibody–antigen interactions monitored via transient ionic current recording using nanopore sensors†

Yi-Lun Ying, ORCID logo a   Ru-Jia Yu, ORCID logo a   Yong-Xu Hu, ORCID logo a   Rui Gao ORCID logo a  and  Yi-Tao Long ORCID logo *a  

* Corresponding authors

a Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry & School of Chemistry & Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. R. China
E-mail: ytlong@ecust.edu.cn

Abstract

Understanding the single molecular protein–protein interaction has great significance in evaluating the affinity of a specific antibody. Herein, the interaction between single α-fetal protein (AFP) and its antibody was monitored via transient ionic current recording by using the antibody functionalized nanopore sensors. More importantly, the kinetic evaluation was performed at the single molecule level to determine the dissociation constant of this interaction. This method enables the monitoring of the kinetic antigen–antibody interaction in their heterogenetic state without any labelling. Our results provided new insights into the evaluation of the antibody's binding affinity and more into the development of immunoassays for diagnostics.

Graphical abstract: Single antibody–antigen interactions monitored via transient ionic current recording using nanopore sensors

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Article information

DOI
https://doi.org/10.1039/C7CC03927A
Article type
Communication
Submitted
21 May 2017
Accepted
06 Jul 2017
First published
06 Jul 2017

Chem. Commun., 2017,53, 8620-8623

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Single antibody–antigen interactions monitored via transient ionic current recording using nanopore sensors

Y. Ying, R. Yu, Y. Hu, R. Gao and Y. Long, Chem. Commun., 2017, 53, 8620 DOI: 10.1039/C7CC03927A

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