Issue 9, 2017, Issue in Progress
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RSC Advances

Magnetic paper – based ELISA for IgM-dengue detection†

G. A. Ortega,ab   S. Pérez-Rodríguezc  and  E. Reguera ORCID logo *a  

* Corresponding authors

a Center for Applied Science and Advanced Technology of IPN, Legaria Unit, Mexico City, Mexico
E-mail: edilso.reguera@gmail.com

b University of Havana, Faculty of Chemistry, Department of Inorganic Chemistry, Havana, Cuba

c National Autonomous University of Mexico, Biomedical Research Institute, Mexico City, Mexico

Abstract

This contribution reports a novel “Magnetic Paper – Based ELISA” using core–shell magnetite@polydopamine nanoparticles supported on a Whatman paper-like new solid immunoassay platform specifically for IgM-dengue antibodies recognition as the proof-of-concept target for antibodies isotype IgM detection. Affordable procedures to deposit magnetite nanoparticles on cellulose paper sheets (Whatman type-1 and ss903) and to conjugate such nanoparticles with anti human-IgM antibodies using polydopamine as linker are reported. Structural features, magnetic behavior, coating homogeneity, and the nanoparticles/linked antibodies ratio were determined. Additionally, “Magnetic Paper – Based ELISA” for IgM-dengue antibodies detection provides a system with improved analytical response (two orders more sensitive with a 700 times lower limit of detection (LOD) than traditional ELISA or using magnetic beads without depositing), appropriate accuracy for real sample detection, low cost, easy manufacturing, and effortless and easy handling.

Graphical abstract: Magnetic paper – based ELISA for IgM-dengue detection

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Article information

DOI
https://doi.org/10.1039/C6RA25992H
Article type
Paper
Submitted
28 Oct 2016
Accepted
20 Dec 2016
First published
17 Jan 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 4921-4932

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Magnetic paper – based ELISA for IgM-dengue detection

G. A. Ortega, S. Pérez-Rodríguez and E. Reguera, RSC Adv., 2017, 7, 4921 DOI: 10.1039/C6RA25992H

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