Issue 50, 2016

An optimized purification process for porcine gastric mucin with preservation of its native functional properties

Abstract

Purified gastric mucins are currently used for a wide range of applications e.g. as a model system for native mucus, as lubricants or antiviral/antibacterial supplements. However, commercially available porcine gastric mucins (PGM) do not exhibit gel-forming properties and show only greatly reduced anti-viral/anti-bacterial activity. Thus, we established a robust purification process for PGM, maintaining its desired properties such as lubricity, gel formation and the selective binding of molecules. We optimized the process in terms of yield and productivity and evaluated the influence of different buffer conditions on mucin quality. Cross-flow filtration using 100 kDa membranes was introduced and optimized to pre-concentrate the mucin solution prior to size exclusion chromatography. A conductivity of less than 100 μS cm−1 after diafiltration was found to be crucial for gel formation. The mucin yield of the optimized process was 66%. The scale-up resulted in a productivity of 0.15 mg purified mucin per mL crude mucus an hour. In total, approx. 65 mg mucin could be purified from one pig stomach. Tribological studies, rheological measurements and co-localization experiments confirmed the retained functionality of purified mucin in terms of lubricity, gel formation and binding interactions with charged molecules, respectively.

Graphical abstract: An optimized purification process for porcine gastric mucin with preservation of its native functional properties

Supplementary files

Article information

Article type
Paper
Submitted
21 Mar 2016
Accepted
23 Apr 2016
First published
26 Apr 2016
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2016,6, 44932-44943

An optimized purification process for porcine gastric mucin with preservation of its native functional properties

V. J. Schömig, B. T. Käsdorf, C. Scholz, K. Bidmon, O. Lieleg and S. Berensmeier, RSC Adv., 2016, 6, 44932 DOI: 10.1039/C6RA07424C

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