Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

Semih Sevim; Sevil Ozer; Luying Feng; Joel Wurzel; Arielle Fakhraee; Naveen Shamsudhin; Bumjin Jang; Carlos Alcantara; Olgaç Ergeneman; Eva Pellicer; Jordi Sort; Tessa Lühmann; Salvador Pané; Bradley J. Nelson; Hamdi Torun

doi:10.1039/C6NH00134C

Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

Semih Sevim,†^a Sevil Ozer,†^a Luying Feng,^a Joel Wurzel,^b Arielle Fakhraee,^c Naveen Shamsudhin,^d Bumjin Jang,^d Carlos Alcantara,^d Olgaç Ergeneman,^d Eva Pellicer,^e Jordi Sort,^f Tessa Lühmann,^b Salvador Pané,*^d Bradley J. Nelson^d and Hamdi Torun

*^a

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* Corresponding authors

^a Department of Electrical and Electronics Engineering, Bogazici University, Bebek, 34342 Istanbul, Turkey
E-mail: hamdi.torun@boun.edu.tr

^b Institute of Pharmacy and Food Chemistry, University of Würzburg, Würzburg, Germany

^c Aeon Scientific AG, Schlieren, Zurich, Switzerland

^d Multi Scale Robotics Lab, Institute of Robotics and Intelligent Systems, ETH Zurich, Zurich, Switzerland
E-mail: vidalp@ethz.ch

^e Departament de Física, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain

^f Institució Catalana de Recerca i Estudis Avançats (ICREA) and Departament de Física, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain

Abstract

We report a novel atomic force microscopy (AFM) technique with dual actuation capabilities using both piezo and magnetic bead actuation for advanced single-molecule force spectroscopy experiments. The experiments are performed by manipulating magnetic microbeads using an electromagnet against a stationary cantilever. Magnetic actuation has been demonstrated before to actuate cantilevers, but here we keep the cantilever stationary and accomplish actuation via free-manipulated microstructures. The developed method benefits from significant reduction of drift, since the experiments are performed without a substrate contact and the measured force is inherently differential. In addition, shrinking the size of the actuator can minimize hydrodynamic forces affecting the cantilever. The new method reported herein allows for the application of constant force to perform force-clamp experiments without any active feedback, profiled for a deeper understanding of biomolecular interactions.

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Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

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Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

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