Issue 41, 2016

Fluorescence “on–off–on” peptide-based chemosensor for the selective detection of Cu2+ and S2− and its application in living cell bioimaging

Abstract

Copper ions are known to be very important for homeostasis, which is critical for the metabolism and development of living organisms. In addition, sulfide ions, as an important endogenously produced gasotransmitter, have been proved to be implicated in a variety of physiological functions such as anti-apoptosis, vasodilation, antioxidation, and anti-inflammation. Herein, we report the development of a novel fluorescence chemosensor (L) based on a tetra-peptide conjugated with dansyl groups as a promising analytical tool for detecting Cu2+ and S2− in 100% aqueous solutions, which exhibits excellent cell biotoxicity and intracellular biosensing ability. The chemosensor L displays an “on–off–on” response type fluorescence change upon the addition of Cu2+ and S2− to aqueous media and living cells. Moreover, L displays high selectivity and sensitivity with the detection limits for Cu2+ and S2− measured to be 88 nM and 75 nM, respectively. This study raises the new possibility of a highly selective and sensitive peptide-based fluorescence chemosensor for multifunctional detection, including cation and anions, using a successive fluorescence response strategy in environmental and biological systems.

Graphical abstract: Fluorescence “on–off–on” peptide-based chemosensor for the selective detection of Cu2+ and S2− and its application in living cell bioimaging

Supplementary files

Article information

Article type
Paper
Submitted
25 Aug 2016
Accepted
07 Sep 2016
First published
07 Sep 2016

Dalton Trans., 2016,45, 16246-16254

Fluorescence “on–off–on” peptide-based chemosensor for the selective detection of Cu2+ and S2− and its application in living cell bioimaging

P. Wang, J. Wu, P. Su, C. Xu, Y. Ge, D. Liu, W. Liu and Y. Tang, Dalton Trans., 2016, 45, 16246 DOI: 10.1039/C6DT03330J

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