Issue 22, 2016
From the journal:

Chemical Society Reviews

A practical guide to working with H2S at the interface of chemistry and biology

Matthew D. Hartlea  and  Michael D. Pluth*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, Materials Science Institute, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
E-mail: pluth@uoregon.edu

Abstract

Hydrogen sulfide (H2S) is the most recently accepted endogenously produced gasotransmitter and is now implicated in a variety of physiological functions. In this tutorial review, our goal is to provide researchers new to the field of H2S chemical biology with practical considerations, pitfalls, and best practices to enable smooth entry into investigations focused on biological H2S. We present practical handling and safety considerations for working with this reactive biomolecule, and cover basic roles of H2S biogenesis and action. Experimental methods for modulating H2S levels, including enzymatic knockout, RNA silencing, enzymatic inhibition, and use of small molecule H2S donors are highlighted. Complementing H2S modulation techniques, we also highlight current strategies for H2S detection and quantification.

Graphical abstract: A practical guide to working with H2S at the interface of chemistry and biology

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Article information

DOI
https://doi.org/10.1039/C6CS00212A
Article type
Tutorial Review
Submitted
14 Mar 2016
First published
11 May 2016
This article is Open Access
Creative Commons BY-NC license

Chem. Soc. Rev., 2016,45, 6108-6117

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A practical guide to working with H2S at the interface of chemistry and biology

M. D. Hartle and M. D. Pluth, Chem. Soc. Rev., 2016, 45, 6108 DOI: 10.1039/C6CS00212A

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