Issue 17, 2016
From the journal:

Chemical Communications

Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags†

Yuling Wang,‡*a   Eugene J. H. Wee‡a  and  Matt Trau*ab  

* Corresponding authors

a Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Roads (Bldg 75), Brisbane, QLD 4072, Australia
E-mail: y.wang27@uq.edu.au, m.trau@uq.edu.au

b School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia

Abstract

Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies.

Graphical abstract: Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags

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Article information

DOI
https://doi.org/10.1039/C6CC00547K
Article type
Communication
Submitted
20 Jan 2016
Accepted
25 Jan 2016
First published
25 Jan 2016

Chem. Commun., 2016,52, 3560-3563

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Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags

Y. Wang, E. J. H. Wee and M. Trau, Chem. Commun., 2016, 52, 3560 DOI: 10.1039/C6CC00547K

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