Issue 10, 2016
From the journal:

Biomaterials Science

In vitro detection of human breast cancer cells (SK-BR3) using herceptin-conjugated liquid crystal microdroplets as a sensing platform

Wang Ding,a   Kailash Chandra Gupta,ac   Soo-Young Park,a   Young-Kyoo Kimb  and  Inn-Kyu Kang*a  

* Corresponding authors

a Department of Polymer Science and Engineering, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 702-701, South Korea
E-mail: ikkang@knu.ac.kr, psy@knu.ac.kr, wangding0420@gmail.com
Fax: +82-53-950-6623
Tel: +82-53-950-5629

b Organic Electronic Laboratory, Department of Chemical Engineering, Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu 702-701, South Korea
E-mail: ykimm@knu.ac.kr

c Polymer Research Laboratory, Department of Chemistry, I.I.T. Roorkee, Roorkee 247667, India
E-mail: kcgptfcy@iitr.ac.in
Fax: +91 1332 273560
Tel: +91 1332 285325

Abstract

The present study utilizes antibody–protein interactions to develop an LC microdroplet based biosensor for naked eye detection of SK-BR3 human breast cancer cells. The herceptin antibody-conjugated LC microdroplets were fabricated using 4-cyano-4′-pentyl biphenyl (5CB) as the liquid crystalline phase and sodium dodecyl sulfate (SDS) as the surfactant. The poly (styrene-b-acrylic acid) amphiphilic block copolymer (PS-b-PA) played a role as a modifier for the liquid crystalline interfaces. The 5CB molecules in the herceptin antibody-conjugated LC microdroplets have shown an orientation transition from radial to bipolar on selective interactions with targeted SK-BR3 breast cancer cells, which are over expressed by the human epidermal growth factor receptor 2-positive (HER2). The herceptin antibody-conjugated LC microdroplets are found to be highly selective in the detection of SK-BR3 cancer cells in the presence of control cells, such as KB cancer cells and fibroblast (FB), and also in the presence of 10% human blood plasma. The interaction forces of the SK-BR3 cancer cells were only effective in causing orientation transitions in 5CB molecules in the LC microdroplets, which clearly suggested that the herceptin antibody-conjugated LC microdroplets could be used as a selective biosensor for a real-time detection of SK-BR3 cancer cells in biological fluids.

Graphical abstract: In vitro detection of human breast cancer cells (SK-BR3) using herceptin-conjugated liquid crystal microdroplets as a sensing platform

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Article information

DOI
https://doi.org/10.1039/C6BM00404K
Article type
Paper
Submitted
15 Jun 2016
Accepted
01 Aug 2016
First published
17 Aug 2016

Biomater. Sci., 2016,4, 1473-1484

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In vitro detection of human breast cancer cells (SK-BR3) using herceptin-conjugated liquid crystal microdroplets as a sensing platform

W. Ding, K. C. Gupta, S. Park, Y. Kim and I. Kang, Biomater. Sci., 2016, 4, 1473 DOI: 10.1039/C6BM00404K

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