Issue 8, 2015
From the journal:

Chemical Science

How to split a G-quadruplex for DNA detection: new insight into the formation of DNA split G-quadruplex†

Jinbo Zhu,ab   Libing Zhang,a   Shaojun Donga  and  Erkang Wang*a  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China
E-mail: ekwang@ciac.ac.cn

b University of Chinese Academy of Sciences, Beijing, P. R. China

Abstract

Here, we get a new insight into the formation of a split G-quadruplex from the viewpoints of the split mode and guanine base number. An unusual result is that the split mode 4 : 8 performed best in six split modes, including the frequently used mode 1 : 3 and 2 : 2 in the split G-quadruplex enhanced fluorescence assay. Circular dichroism spectra verified the conclusion. The application of the split G-quadruplex based assay in DNA detection was performed on the point mutations of the JAK2 V617F and HBB genes. A multi-target analysis method based on a pool of G-segments split from T30695 (GGGTGGGTGGGTGGGT) by the magic “law of 4 : 8” was established.

Graphical abstract: How to split a G-quadruplex for DNA detection: new insight into the formation of DNA split G-quadruplex

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Article information

DOI
https://doi.org/10.1039/C5SC01287B
Article type
Edge Article
Submitted
09 Apr 2015
Accepted
31 May 2015
First published
02 Jun 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2015,6, 4822-4827

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How to split a G-quadruplex for DNA detection: new insight into the formation of DNA split G-quadruplex

J. Zhu, L. Zhang, S. Dong and E. Wang, Chem. Sci., 2015, 6, 4822 DOI: 10.1039/C5SC01287B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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