Issue 13, 2016
From the journal:

Chemical Communications

Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis–mass spectrometry†

Fang Yuan,a   Xiao-Hui Zhang,a   Ji Nie,a   Hong-Xu Chen,b   Ying-Lin Zhou*a  and  Xin-Xiang Zhang*a  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China
E-mail: zhouyl@pku.edu.cn, zxx@pku.edu.cn
Fax: +86-10-62754112
Tel: +86-10-62754112

b Shanghai AB Sciex Analytical Instrument Trading Co., Ltd, Beijing, P. R. China

Abstract

A newly developed sheathless interface for capillary electrophoresis–mass spectrometry, using a porous tip sprayer, was first applied for highly sensitive determination of cytosine modifications. The system performed well in identification and quantification of both 5-methylcytosine and 5-hydroxymethylcytosine using only 125 pg (∼20 cells) genomic DNA samples.

Graphical abstract: Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis–mass spectrometry

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Article information

DOI
https://doi.org/10.1039/C5CC10155G
Article type
Communication
Submitted
10 Dec 2015
Accepted
04 Jan 2016
First published
04 Jan 2016

Chem. Commun., 2016,52, 2698-2700

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Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis–mass spectrometry

F. Yuan, X. Zhang, J. Nie, H. Chen, Y. Zhou and X. Zhang, Chem. Commun., 2016, 52, 2698 DOI: 10.1039/C5CC10155G

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