Issue 100, 2015
From the journal:

Chemical Communications

A programmable Y-shaped junction scaffold-mediated modular and cascade amplification strategy for the one-step, isothermal and ultrasensitive detection of target DNA†

Shufeng Liu,*a   Hongwei Gong,a   Xinya Sun,a   Tao Liua  and  Li Wanga  

* Corresponding authors

a Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China
E-mail: sliu@qust.edu.cn

Abstract

The programmable DNA polymerization across the two branches of the assembled Y-shaped junction was ingeniously manipulated for modular target recycling and cascade lambda exonuclease cleavage, which afforded the one-pot, isothermal and ultrasensitive detection of target DNA. A low detection limit of 28.2 fM of target DNA with an excellent selectivity could be obtained.

Graphical abstract: A programmable Y-shaped junction scaffold-mediated modular and cascade amplification strategy for the one-step, isothermal and ultrasensitive detection of target DNA

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Article information

DOI
https://doi.org/10.1039/C5CC07659E
Article type
Communication
Submitted
13 Sep 2015
Accepted
15 Oct 2015
First published
15 Oct 2015

Chem. Commun., 2015,51, 17756-17759

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A programmable Y-shaped junction scaffold-mediated modular and cascade amplification strategy for the one-step, isothermal and ultrasensitive detection of target DNA

S. Liu, H. Gong, X. Sun, T. Liu and L. Wang, Chem. Commun., 2015, 51, 17756 DOI: 10.1039/C5CC07659E

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