Issue 62, 2015
From the journal:

Chemical Communications

Double-site recognition of pathogenic bacterial whole cells based on an antibiotic-affinity strategy†

Hongfei Gao,a   Shijia Yang,a   Jing Han,b   Jie Xiong,a   Weijun Kong,a   Chong Li,a   Guojian Liaoa  and  Zhifeng Fu*a  

* Corresponding authors

a Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China
E-mail: fuzf@swu.edu.cn
Tel: +86-23-68250184

b Department of Pharmacy, People's Hospital of Gansu Province, Lanzhou 730000, China

Abstract

An antibiotic-affinity strategy was designed to detect pathogenic bacterial whole cells based on the strong affinity of the antibiotic agent to bind to the cell wall of the bacterium. Vancocin, a powerful glycopeptide antibiotic, was used as a molecular recognition agent for Staphylococcus aureus. To improve its specificity, IgG was utilized as the second recognition agent based on the binding between the Fc region of IgG and protein A in the cell wall.

Graphical abstract: Double-site recognition of pathogenic bacterial whole cells based on an antibiotic-affinity strategy

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC02814K
Article type
Communication
Submitted
04 Apr 2015
Accepted
15 Jun 2015
First published
16 Jun 2015

Chem. Commun., 2015,51, 12497-12500

Permissions

Request permissions

Double-site recognition of pathogenic bacterial whole cells based on an antibiotic-affinity strategy

H. Gao, S. Yang, J. Han, J. Xiong, W. Kong, C. Li, G. Liao and Z. Fu, Chem. Commun., 2015, 51, 12497 DOI: 10.1039/C5CC02814K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements