Issue 33, 2015
From the journal:

Chemical Communications

Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA†

Jun Gao,‡a   Boris L. Zybailov,‡a   Alicia K. Byrd,a   Wezley C. Griffin,a   Shubeena Chib,a   Samuel G. Mackintosh,a   Alan J. Tacketta  and  Kevin D. Raney*a  

* Corresponding authors

a Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 516, Little Rock, AR 72205, USA
E-mail: RaneyKevinD@uams.edu
Fax: +1 (501) 686-8169
Tel: +1 (501) 686-5244

Abstract

Using a G-quadruplex bait, we identified the transcription co-activator Sub1 as a G-quadruplex binding protein by quantitative LC-MS/MS and demonstrated in vivo G-quadruplex binding by ChIP. In vitro, Sub1, and its human homolog PC4, bind preferentially to G-quadruplexes. This provides a possible mechanism by which G-quadruplexes can influence gene transcription.

Graphical abstract: Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA

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Article information

DOI
https://doi.org/10.1039/C5CC00742A
Article type
Communication
Submitted
05 Feb 2015
Accepted
18 Mar 2015
First published
19 Mar 2015

Chem. Commun., 2015,51, 7242-7244

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Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA

J. Gao, B. L. Zybailov, A. K. Byrd, W. C. Griffin, S. Chib, S. G. Mackintosh, A. J. Tackett and K. D. Raney, Chem. Commun., 2015, 51, 7242 DOI: 10.1039/C5CC00742A

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