Issue 10, 2016
From the journal:

Analytical Methods

Development of an analytical method by defining a design space: a case study of saponin determination for Panax notoginseng extracts

Xingchu Gong,a   Ying Zhang,a   Huali Chen,a   Teng Chen,a   Jianyang Pan,a   Xiaoyu Wanga  and  Haibin Qu*a  

* Corresponding authors

a Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
E-mail: quhb@zju.edu.cn

Abstract

A reversed-phase HPLC-UV method was developed using a design space approach for the simultaneous determination of five saponins in the extracts of Panax notoginseng, which were notoginsenoside R1, ginsenosides Rg1, Re, Rb1, and Rd. In the development of this method, resolutions of three critical peak pairs were considered as critical quality attributes (CQAs). The effects of four parameters on gradient elution were quantitatively investigated. Design space with a probability to attain CQA criteria higher than 0.9 was calculated using a Monte-Carlo simulation method. Several gradients were optimized in this work. The newly developed method was validated and can be used for saponin concentration determination of Panax notoginseng extracts.

Graphical abstract: Development of an analytical method by defining a design space: a case study of saponin determination for Panax notoginseng extracts

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Article information

DOI
https://doi.org/10.1039/C5AY03119B
Article type
Paper
Submitted
30 Nov 2015
Accepted
10 Feb 2016
First published
11 Feb 2016

Anal. Methods, 2016,8, 2282-2289

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Development of an analytical method by defining a design space: a case study of saponin determination for Panax notoginseng extracts

X. Gong, Y. Zhang, H. Chen, T. Chen, J. Pan, X. Wang and H. Qu, Anal. Methods, 2016, 8, 2282 DOI: 10.1039/C5AY03119B

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