Issue 18, 2015
From the journal:

Analyst

DNA methylation detection with end-to-end nanorod assembly-enhanced surface plasmon resonance†

Xuemei Li,*a   Ting Songb  and  Xilin Guob  

* Corresponding authors

a School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China

b Center of Cooperative Innovation for Chemical Imaging Functional Probes in Universities of Shandong, College of Chemistry, Shandong Normal University, Jinan 250014, P.R. China
E-mail: xuemei_li@yeah.net
Fax: +86-539-8766867
Tel: +86-539-8766867

Abstract

The Au nanorod (AuNR) assembly-enhanced surface plasmon resonance (SPR) system coupling with polymerization and nicking reactions was developed for amplified detection of DNA methylation and adenine methylation (Dam) methyltransferase (MTase) activity assay. The biosensor showed a good linear relationship between the SPR angle shift and the Dam MTase concentration over a range from 0.5 to 120 U mL−1, with a detection limit of 0.2 U mL−1. This study provided a sensitive platform to screen inhibitors for Dam MTase with great potential to be further applied in early clinical diagnosis.

Graphical abstract: DNA methylation detection with end-to-end nanorod assembly-enhanced surface plasmon resonance

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Article information

DOI
https://doi.org/10.1039/C5AN01015B
Article type
Communication
Submitted
20 May 2015
Accepted
26 Jul 2015
First published
27 Jul 2015

Analyst, 2015,140, 6230-6233

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DNA methylation detection with end-to-end nanorod assembly-enhanced surface plasmon resonance

X. Li, T. Song and X. Guo, Analyst, 2015, 140, 6230 DOI: 10.1039/C5AN01015B

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