pFe3+ determination of multidentate ligands by a fluorescence assay

Yongmin Ma; Tao Zhou; Robert C. Hider

doi:10.1039/C5AN00123D

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pFe³⁺ determination of multidentate ligands by a fluorescence assay†

Yongmin Ma,^a Tao Zhou^b and Robert C. Hider*^c

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* Corresponding authors

^a College of Pharmaceutical Science, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, P R China

^b College of Food Science and Biotechnology, Engineering, Zhejiang Gongshang University, Hangzhou, P R China

^c Department of Pharmacy, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London, UK
E-mail: Robert.hider@kcl.ac.uk

Abstract

The fluorescence intensity of the iron–CP691 complex in the presence of a competing multidentate ligand is associated with pFe³⁺ of the competing ligand and the relative fluorescence has a linear correlation with the pFe³⁺ values. A correlation was also found to exist between the relative fluorescence and the ratio of a competing ligand to the probe CP691. Based on this assay, the pFe³⁺ value of a range of hexadentate ligands, dendrimers and polymers can be determined when they fall in the range 24.5–30.5. Only small quantities of chelators are required for this assay.

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Article information

DOI: https://doi.org/10.1039/C5AN00123D
Article type: Paper
Submitted: 20 Jan 2015
Accepted: 20 Mar 2015
First published: 23 Mar 2015

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Analyst, 2015,140, 3603-3606

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pFe³⁺ determination of multidentate ligands by a fluorescence assay

Y. Ma, T. Zhou and R. C. Hider, Analyst, 2015, 140, 3603 DOI: 10.1039/C5AN00123D

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