Issue 108, 2014
From the journal:

RSC Advances

Calixarene modified montmorillonite: a novel design for biosensing applications†

Burak Sonmez,a   Serkan Sayin,b   Esra Evrim Yalcinkaya,c   Didem Ag Seleci,a   Huseyin Bekir Yildiz,b   Dilek Odaci Demirkol*ad  and  Suna Timur*ad  

* Corresponding authors

a Ege University Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey
E-mail: suna.timur@ege.edu.tr, dilek.odaci.demirkol@ege.edu.tr

b KTO Karatay University, Department of Materials Science and Nanotechnology Engineering, 42020 Konya, Turkey

c Ege University Faculty of Science, Chemistry Department, 35100 Bornova-Izmir, Turkey

d Ege University, Institute of Drug Abuse Toxicology &Pharmaceutical Sciences, 35100 Bornova-Izmir, Turkey

Abstract

Here we report the synthesis, characterization and application of calixarene (Calix) modified montmorillonite (Mt) as a platform for bio-applications such as biomolecule immobilization and biosensing technologies. This modification enhanced the biomolecule immobilization capability of Mt. Initially, amino-functionalised calixarenes (Calix-NH2) were synthesized and used as a modifier. X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric analysis were performed to verify the modification of the clay minerals. For the biosensor construction, Calix-NH2 modified Mt (Calix-NH2/Mt), bovine serum albumin (BSA), glutaraldehyde (GA) and pyranose oxidase were immobilized on the surface of a glassy carbon electrode which was then referred to as a Calix-NH2/Mt/PyOx biosensor. After optimization of the enzyme amount and pH, analytical characteristics were investigated in detail.

Graphical abstract: Calixarene modified montmorillonite: a novel design for biosensing applications

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Article information

DOI
https://doi.org/10.1039/C4RA11818A
Article type
Paper
Submitted
05 Oct 2014
Accepted
06 Nov 2014
First published
06 Nov 2014

RSC Adv., 2014,4, 62895-62902

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Calixarene modified montmorillonite: a novel design for biosensing applications

B. Sonmez, S. Sayin, E. E. Yalcinkaya, D. A. Seleci, H. B. Yildiz, D. O. Demirkol and S. Timur, RSC Adv., 2014, 4, 62895 DOI: 10.1039/C4RA11818A

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