Issue 54, 2014

A quantum dot-based microRNA nanosensor for point mutation assays

Abstract

We have developed a quantum dot-based microRNA nanosensor for point mutation assays using primer generation-mediated rolling circle amplification. The proposed method exhibits high sensitivity with a detection limit of as low as 50.9 aM and a large dynamic range of 7 orders of magnitude from 0.1 fM to 1 nM. Importantly, this method can be further applied to analyze the point mutation of mir-196a2 in the lung tissues of non small-cell lung cancer patients.

Graphical abstract: A quantum dot-based microRNA nanosensor for point mutation assays

Supplementary files

Article information

Article type
Communication
Submitted
19 Mar 2014
Accepted
14 Apr 2014
First published
15 Apr 2014

Chem. Commun., 2014,50, 7160-7162

A quantum dot-based microRNA nanosensor for point mutation assays

Y. Zeng, G. Zhu, X. Yang, J. Cao, Z. Jing and C. Zhang, Chem. Commun., 2014, 50, 7160 DOI: 10.1039/C4CC02034K

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