Issue 38, 2013
From the journal:

Journal of Materials Chemistry B

Highly dispersible PEGylated graphene/Au composites as gene delivery vector and potential cancer therapeutic agent†

Fang-Fang Cheng,a   Wei Chen,b   Li-Hui Hu,a   Gang Chen,a   Hai-Tao Miao,a   Chenzhong Lic  and  Jun-Jie Zhu*a  

* Corresponding authors

a State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, P.R.China
E-mail: jjzhu@nju.edu.cn
Fax: +86-25-8359-4976
Tel: +86-25-8359-4976

b State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing 210093, China

c Department of Biomedical Engineering, Florida International University, 10555 W. Flagler St., Miami, Florida 33174, USA

Abstract

Graphene/Au composites with a high positive charge, which is advantageous for the binding and condensation of negatively charged siRNA, are synthesized via an in situ reduction method, using PEI as a reductant and protective reagent. Owing to the sufficient amounts of amino groups, PEI-grafted graphene/Au composites can be further modified with methoxyl-PEG to acquire low cytotoxicity, novel blood compatibility, and optimal dispersibility in physiological environments. The obtained PEGylated PEI-grafted graphene/Au composites (PPGA) allow efficient loading of siRNA, forming PPGA/siRNA complexes to transport into HL-60 cells and downregulated anti-apoptosis Bcl-2 protein, indicating PPGA is a suitable platform for gene delivery. Moreover, PPGA display an enhanced photothermal response with respect to PPG under NIR laser irradiation, suggesting that PPGA can be used as an efficient photothermal agent.

Graphical abstract: Highly dispersible PEGylated graphene/Au composites as gene delivery vector and potential cancer therapeutic agent

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Article information

DOI
https://doi.org/10.1039/C3TB20656D
Article type
Paper
Submitted
09 May 2013
Accepted
22 Jul 2013
First published
22 Jul 2013

J. Mater. Chem. B, 2013,1, 4956-4962

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Highly dispersible PEGylated graphene/Au composites as gene delivery vector and potential cancer therapeutic agent

F. Cheng, W. Chen, L. Hu, G. Chen, H. Miao, C. Li and J. Zhu, J. Mater. Chem. B, 2013, 1, 4956 DOI: 10.1039/C3TB20656D

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