In situ visualization and detection of protein sulfenylation responses in living cells through a dimedone-based fluorescent probe

Qin Yin; Chusen Huang; Chao Zhang; Weiping Zhu; Yufang Xu; Xuhong Qian; Yi Yang

doi:10.1039/C3OB41434E

In situ visualization and detection of protein sulfenylation responses in living cells through a dimedone-based fluorescent probe†

Qin Yin,‡^a Chusen Huang,‡^ab Chao Zhang,^a Weiping Zhu,*^a Yufang Xu,*^a Xuhong Qian^a and Yi Yang*^a

* Corresponding authors

^a State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China
E-mail: wpzhu@ecust.edu.cn, yfxu@ecust.edu.cn, yiyang@ecsut.edu.cn
Fax: (+86) 21-64252603

^b Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, PR China

Abstract

Sulfenylation is one of the reversible post-translational modifications, playing significant roles in cellular redox homeostasis and signaling systems. Herein, small fluorescent probe (CPD and CPDDM) based live-cell labelling technology for the visualization of protein sulfenylation responses in living cells has been developed. This approach enables the detection of protein sulfenylation without the need for cell lysis, fixation or purification, and permits the noninvasive study of protein sulfenylation in live cells through the direct fluorescent readout. This technology also can realize dynamic tracking of protein sulfenylation in situ with minimal perturbation to sulfenylated proteins and less interference with cellular function. Information on the global distribution and dynamic changes of endogenous protein sulfenylation has been obtained.

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DOI: https://doi.org/10.1039/C3OB41434E
Article type: Paper
Submitted: 12 Jul 2013
Accepted: 17 Sep 2013
First published: 17 Sep 2013

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Org. Biomol. Chem., 2013,11, 7566-7573

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In situ visualization and detection of protein sulfenylation responses in living cells through a dimedone-based fluorescent probe

Q. Yin, C. Huang, C. Zhang, W. Zhu, Y. Xu, X. Qian and Y. Yang, Org. Biomol. Chem., 2013, 11, 7566 DOI: 10.1039/C3OB41434E

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Organic & Biomolecular Chemistry

In situ visualization and detection of protein sulfenylation responses in living cells through a dimedone-based fluorescent probe†

Abstract

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In situ visualization and detection of protein sulfenylation responses in living cells through a dimedone-based fluorescent probe

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