Issue 16, 2013
From the journal:

Analytical Methods

Frontal elutionpaper chromatography for ambient ionization mass spectrometry: analyzing powder samples

Yun-Qing Huang,†a   Jin-Qing You,†b   Yupeng Cheng,c   Wenjian Sun,c   Li Dingc  and  Yu-Qi Feng*a  

* Corresponding authors

a Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China
E-mail: yqfeng@whu.edu.cn
Fax: +86-27-68755595
Tel: +86-27-68755595

b China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou 310008, China

c Shimadzu Research Laboratory (Shanghai) Company, Ltd., Shanghai 201201, China

Abstract

We developed a convenient method by coupling frontal elution paper chromatography with desorption corona beam ionization mass spectrometry (FEPC/DCBI-MS) for rapid analysis of powder samples. In this method, the powder was deposited directly onto a coarse strip near the base of a triangular paper sheet, and then a strong elution solvent was pipetted onto the base of the paper sheet in the open air for developing. The sample zone migrated at the solvent front under strong elution conditions. Target analytes were finally condensed at the V-shaped tip which was then placed under the visible plasma beam of DCBI for ionization. Steps of solvent extraction and developing were performed on a paper sheet simultaneously. The overall procedure requires less than two minutes. Signal intensities of target analytes were improved significantly due to analyte condensation at the tip and matrix effect reduction. Fundamentals and applications of FEPC/DCBI-MS were demonstrated by analysis of chlorphenamine in herbal medicines, clenbuterol in pig feed powder and nicotine in house-hold dust. The limits of detection ranged from 0.35 μg g−1 to 1.5 μg g−1 (0.52–2.2 ng, absolute) in full-scan positive-ion mode. The linear range was 5.0 μg g−1 to 5.0 × 102 μg g−1 with satisfactory linear coefficient (R2 = 0.885–0.956). Good reproducibility was achieved with relative standard deviations (RSDs) less than 20% and the recoveries ranged from 70 to 90%. The results demonstrate that the improved FEPC-DCBI-MS method is satisfactory for semi-quantitative evaluation of analytes in powder samples.

Graphical abstract: Frontal elution paper chromatography for ambient ionization mass spectrometry: analyzing powder samples

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C3AY40336J
Article type
Paper
Submitted
02 Mar 2013
Accepted
13 Jun 2013
First published
13 Jun 2013

Anal. Methods, 2013,5, 4105-4111

Permissions

Request permissions

Frontal elution paper chromatography for ambient ionization mass spectrometry: analyzing powder samples

Y. Huang, J. You, Y. Cheng, W. Sun, L. Ding and Y. Feng, Anal. Methods, 2013, 5, 4105 DOI: 10.1039/C3AY40336J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements