Issue 10, 2013
From the journal:

Analyst

Hydrophobicity-driven self-assembly of protein and silver nanoparticles for protein detection using surface-enhanced Raman scattering†

Mehmet Kahraman,*ab   Ben N. Balzac  and  Sebastian Wachsmann-Hogiu*ad  

* Corresponding authors

a Center for Biophotonics Science and Technology, University of California Davis, Sacramento, CA, USA
E-mail: mkahraman@ucdavis.edu

b Department of Chemistry, Faculty of Arts and Sciences, University of Gaziantep, 27310 Sehitkamil/Gaziantep, Turkey
E-mail: mkahraman@gantep.edu.tr

c Bielefeld University, Bielefeld, Germany

d Department of Pathology and Laboratory Medicine, University of California Davis, Sacramento, CA, USA
E-mail: swachsmann@ucdavis.edu

Abstract

Surface-enhanced Raman scattering (SERS) is a promising analytical technique for the detection and characterization of biological molecules and structures. The role of hydrophobic and hydrophilic surfaces in the self-assembly of protein-metallic nanoparticle structures for label-free protein detection is demonstrated. Aggregation is driven by both the hydrophobicity of the surface as well as the charge of the proteins. The best conditions for obtaining a reproducible SERS signal that allows for sensitive, label-free protein detection are provided by the use of hydrophobic surfaces and 16 × 1011 NPs per mL. A detection limit of approximately 0.5 μg mL−1 is achieved regardless of the proteins' charge properties and size. The developed method is simple and can be used for reproducible and sensitive detection and characterization of a wide variety of biological molecules and various structures with different sizes and charge status.

Graphical abstract: Hydrophobicity-driven self-assembly of protein and silver nanoparticles for protein detection using surface-enhanced Raman scattering

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Article information

DOI
https://doi.org/10.1039/C3AN00025G
Article type
Paper
Submitted
04 Jan 2013
Accepted
03 Mar 2013
First published
05 Mar 2013

Analyst, 2013,138, 2906-2913

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Hydrophobicity-driven self-assembly of protein and silver nanoparticles for protein detection using surface-enhanced Raman scattering

M. Kahraman, B. N. Balz and S. Wachsmann-Hogiu, Analyst, 2013, 138, 2906 DOI: 10.1039/C3AN00025G

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