Click chemistry: a new facile and efficient strategy for the preparation of Fe3O4 nanoparticles covalently functionalized with IDA-Cu and their application in the depletion of abundant protein in blood samples

Guiqin Jian; Yuxing Liu; Xiwen He; Langxing Chen; Yukui Zhang

doi:10.1039/C2NR31430D

Click chemistry: a new facile and efficient strategy for the preparation of Fe₃O₄nanoparticles covalently functionalized with IDA-Cu and their application in the depletion of abundant protein in blood samples

Guiqin Jian,^ab Yuxing Liu,^ab Xiwen He,^ab Langxing Chen*^ab and Yukui Zhang*^abc

Author affiliations

* Corresponding authors

^a State Key Laboratory of Medical Chemical Biology, Nankai University, Tianjin 300071, China

^b Department of Chemistry, Nankai University, Tianjin 300071, P. R. China
E-mail: lxchen@nankai.edu.cn
Fax: +86 22 23502458

^c Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China
E-mail: ykzhang@dicp.ac.cn

Abstract

In this study, we report a novel method to synthesize core–shell structured Fe₃O₄ nanoparticles (NPs) covalently functionalized with iminodiacetic acid (IDA) via click chemistry between the azide and alkyne groups and charged with Cu²⁺. Firstly, the Fe₃O₄@SiO₂ NPs were obtained using tetraethoxysilane (TEOS) to form a silica shell on the surface of the Fe₃O₄ core. The azide group-modified Fe₃O₄@SiO₂ NPs were obtained by a sol–gel process using 3-azidopropyltriethoxysilane (AzPTES) as the silane agent. Fe₃O₄@SiO₂–N₃ was directly reacted with N-propargyl iminodiacetic via click chemistry, in the presence of a Cu(I) catalyst, to acquire the IDA-modified Fe₃O₄ NPs. Finally, through the addition of Cu²⁺, the Fe₃O₄@SiO₂–IDA-Cu NP product was obtained. The morphology, structure and composition of the NPs were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The resulting NPs showed a strong magnetic response to an externally applied magnetic field, a high adsorption capacity and excellent specificity towards hemoglobin (Hb). In addition, the Fe₃O₄@SiO₂–IDA-Cu NPs can be used for the selective removal of abundant Hb protein in bovine and human blood samples.

Article information

https://doi.org/10.1039/C2NR31430D

Article type

Paper

Submitted

08 Jun 2012

Accepted

10 Aug 2012

First published

15 Aug 2012

Download Citation

Nanoscale, 2012,4, 6336-6342

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Click chemistry: a new facile and efficient strategy for the preparation of Fe₃O₄ nanoparticles covalently functionalized with IDA-Cu and their application in the depletion of abundant protein in blood samples

G. Jian, Y. Liu, X. He, L. Chen and Y. Zhang, Nanoscale, 2012, 4, 6336 DOI: 10.1039/C2NR31430D

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