Issue 24, 2012
From the journal:

Analyst

Analysis of flavonoids by graphene-based surface-assisted laser desorption/ionization time-of-flight mass spectrometry†

Chih-Wei Liu,‡a   Min-Wei Chien,‡a   Ching-Yuan Su,b   Hui-Yi Chen,c   Lain-Jong Li*d  and  Chien-Chen Lai*ae  

* Corresponding authors

a Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, Taiwan
E-mail: lailai@dragon.nchu.edu.tw
Fax: +886 422858163
Tel: +886 422840485 ext. 235

b Department of Electronic Engineering, Chang Gung University, Taoyuan 33302, Taiwan

c Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan

d Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan
E-mail: lanceli@gate.sinica.edu.tw
Fax: +886 223620200
Tel: +886 223668205

e Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40227, Taiwan

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple and fast technique for the analysis of large biomolecules but is not suitable for the detection of low molecular weight molecules and compounds, such as flavonoids and phenylpropanoids, mainly due to the lack of an appropriate matrix. Flavonoids and phenylpropanoids, such as coumarin and its derivatives, have attracted much attention recently because of their pharmacological activities and putative therapeutic benefits. In this study, we developed a quick and simple LDI-TOF MS method for the detection of flavonoids and the derivatives of coumarin. Analytes were spotted onto a matrix of graphene-based nanoparticles and then analyzed by LDI-TOF MS in the negative ion mode. Analysis of the sensitivity and effect of different graphene-based nanoparticles including graphene, graphene oxide, and reduced graphene oxide on desorption/ionization of analytes showed that graphene oxide was the most suitable matrix. Moreover, we found that graphene oxide sheets of larger lateral size resulted in better desorption/ionization efficiency. Overall, we show that graphene oxide is a useful matrix for the analysis of flavonoids and the derivatives of coumarin by LDI-TOF MS in the negative ion mode.

Graphical abstract: Analysis of flavonoids by graphene-based surface-assisted laser desorption/ionization time-of-flight mass spectrometry

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C2AN36155H
Article type
Paper
Submitted
19 Aug 2012
Accepted
11 Oct 2012
First published
12 Oct 2012

Analyst, 2012,137, 5809-5816

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Analysis of flavonoids by graphene-based surface-assisted laser desorption/ionization time-of-flight mass spectrometry

C. Liu, M. Chien, C. Su, H. Chen, L. Li and C. Lai, Analyst, 2012, 137, 5809 DOI: 10.1039/C2AN36155H

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