Issue 12, 2011
From the journal:

Nanoscale

Development of a mass-producible on-chip plasmonic nanohole array biosensor†

Kohei Nakamoto,ab   Ryoji Kurita,b   Osamu Niwa,*ab   Toshiyuki Fujiicd  and  Munehiro Nishida*e  

* Corresponding authors

a Institute of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Japan
E-mail: niwa.o@aist.go.jp

b National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Japan

c Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, Japan

d Research Institute for Science and Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan

e Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan
E-mail: mnishida@hiroshima-u.ac.jp

Abstract

We have developed a polymer film based plasmonic device whose optical properties are tuned for measuring biological samples. The device has a circular nanohole array structure fabricated with a nanoimprint technique using a UV curable polymer, and then gold thin film is deposited by electron beam deposition. Therefore, the device is mass-producible, which is also very important for bioaffinity sensors. First the gold film thickness and hole depth were optimized to obtain the maximum dip shift for the reflection spectra. The dip shift is equivalent to the sensitivity to refractive index changes at the plasmonic device surface. We also calculated the variation in reflection spectra by changing the above conditions using the finite-difference time domain method, and we obtained agreement between the theoretical and experimental curves. The nanohole periodicity was adjusted from 400 to 900 nm to make it possible to perform measurements in the visible wavelength region to measure the aqueous samples with less optical absorption. The tuned bottom filled gold nanohole array was incorporated in a microfluidic device covered with a PDMS based microchannel that was 2 mm wide and 20 μm deep. As a proof of concept, the device was used to detect TNF-α by employing a direct immunochemical reaction on the plasmonic array, and a detection limit of 21 ng mL−1 was obtained by amplification with colloidal gold labeling instead of enzymatic amplification.

Graphical abstract: Development of a mass-producible on-chip plasmonic nanohole array biosensor

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Article information

DOI
https://doi.org/10.1039/C1NR10883B
Article type
Paper
Submitted
20 Jul 2011
Accepted
27 Sep 2011
First published
28 Oct 2011

Nanoscale, 2011,3, 5067-5075

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Development of a mass-producible on-chip plasmonic nanohole array biosensor

K. Nakamoto, R. Kurita, O. Niwa, T. Fujii and M. Nishida, Nanoscale, 2011, 3, 5067 DOI: 10.1039/C1NR10883B

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