Issue 28, 2011
From the journal:

Physical Chemistry Chemical Physics

Phospholamban generates cation selective ion channels

Serena Smeazzetto,a   Indra Schröder,b   Gerhard Thiel*b  and  Maria Rosa Moncellia  

* Corresponding authors

a Dipartimento di Chimica, Università di Firenze, Via della Lastruccia 3, 50019 Sesto Fiorentino, Firenze, Italy

b FB Biologie, Technische Universität Darmstadt, Schnittspahnstrasse 3, 64287 Darmstadt, Germany
E-mail: gerhardthiel@me.com
Fax: +49-6151-16-3202
Tel: +49-6151-16-6050

Abstract

Phosholamban (PLN) is involved in the contractility of cardiac muscles by regulating the intracellular calcium concentration (Ca2+cyt) of cardiac myocytes. This occurs via a modulation of the sarco-/endoplasmic CaATPase (SERCA). In spite of high-resolution structures the molecular mode of PLN action is yet not known. In the present paper we readdress the question whether PLN proteins can generate ion channel activity. Reconstitution of PLN in planar lipid bilayers reveals single channel fluctuations, which are characterized by two conductance levels, long open/closed dwell times, moderate selectivity between monovalent cations and no perceivable Ca2+ permeability. The PLN generated channel activity could be inhibited by a PLN antibody (abPLN) implying that the channel activity is indeed due to the inherent channel function of the PLN protein.

Graphical abstract: Phospholamban generates cation selective ion channels

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Article information

DOI
https://doi.org/10.1039/C1CP20460B
Article type
Paper
Submitted
21 Feb 2011
Accepted
25 May 2011
First published
20 Jun 2011

Phys. Chem. Chem. Phys., 2011,13, 12935-12939

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Phospholamban generates cation selective ion channels

S. Smeazzetto, I. Schröder, G. Thiel and M. R. Moncelli, Phys. Chem. Chem. Phys., 2011, 13, 12935 DOI: 10.1039/C1CP20460B

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