Issue 4, 2012
From the journal:

Analyst

Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Zhenyu Lin,*a   Lifen Chen,ab   Guiyun Zhang,a   Qida Liu,c   Bin Qiu,a   Zongwei Caid  and  Guonan Chen*a  

* Corresponding authors

a Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, China
E-mail: zylin@fzu.edu.cn, gnchen@fzu.edu.cn
Fax: (+86)-591-22866135

b State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, China

c MOE Key Laboratory for Strength and Vibration, Xi'an Jiaotong University, China

d Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China

Abstract

A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au–S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10−8 to 1.0 × 10−11 mol L−1, with a detection limit of 2.0 × 10−12 mol L−1. The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

Graphical abstract: Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

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Article information

DOI
https://doi.org/10.1039/C1AN15856B
Article type
Communication
Submitted
14 Sep 2011
Accepted
24 Oct 2011
First published
13 Dec 2011

Analyst, 2012,137, 819-822

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Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol

Z. Lin, L. Chen, G. Zhang, Q. Liu, B. Qiu, Z. Cai and G. Chen, Analyst, 2012, 137, 819 DOI: 10.1039/C1AN15856B

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