Issue 23, 2011

Using ratiometric indicator-displacement assays in semi-quantitative colorimetric determination of chloride, bromide, and iodide anions

Abstract

A ratiometric indicator-displacement assay (RIDA) array has been developed for the semi-quantitative colorimetric determination of chloride, bromide, and iodide anions. Determinations of these halide anions follow the displacement reaction using the chelate compound of (2-(3,5-dibromo-2-pyridylazo)-5-(diethylamino)phenol) (3,5-Br2-PADAP) and heavy metal salts as colorimetric reagent. Different from regular silver nitrate titrations, the chloride, bromide, and iodide anions compete with the 3,5-Br2-PADAP ligand and change the colour of the 3,5-Br2-PADAP–metal chelate compound dramatically. These clearer colour changes make the semi-quantitative colorimetric determination of chloride, bromide, and iodide anions possible. The colour changes are imaged using a conventional flatbed scanner, and digitized. After statistical analysis, these colour changes in the RIDA array provide facile identification of chloride, bromide, and iodide anions at a wide concentration range (10 μM to 10 mM) without any misclassification. The RIDA array is able to discriminate without misclassifications among seven concentrations of chloride, bromide, and iodide anions. No shelf life issue exists because the chelating compounds react with halide anions directly without any pre-immobilizations.

Graphical abstract: Using ratiometric indicator-displacement assays in semi-quantitative colorimetric determination of chloride, bromide, and iodide anions

Supplementary files

Article information

Article type
Paper
Submitted
01 Aug 2011
Accepted
07 Sep 2011
First published
12 Oct 2011

Analyst, 2011,136, 5025-5029

Using ratiometric indicator-displacement assays in semi-quantitative colorimetric determination of chloride, bromide, and iodide anions

Z. Shen, H. Li and L. Feng, Analyst, 2011, 136, 5025 DOI: 10.1039/C1AN15679A

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