Issue 14, 2011
From the journal:

Organic & Biomolecular Chemistry

Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM†

Piyali Dutta,a   Namrata Ray,a   Sarita Roy,b   Anjan Kr. Dasgupta,b   Othman Bouloussac  and  Amitabha Sarkar*a  

* Corresponding authors

a Department of Organic Chemistry, Indian Association for the Cultivation of Science, Kolkata-700032, India
E-mail: ocas@iacs.res.in
Fax: +91 332473280
Tel: +91 3324734971

b Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata-700 019, India

c Institut Curie, Laboratoire Physico-Chimie Curie (UMR CNRS 168), 26 rue d'Ulm, 75248 Paris cedex 05, France

Abstract

A cross-metathesis reaction between an alkene terminated self-assembled monolayer (SAM) on glass/Si wafer and an alkene tethered Fischer carbene complex yielded a functionalized surface. Rapid aminolysis of the Fischer carbene moieties permit efficient anchoring of amine containing molecules on such a surface. Attachment of 1-pyrenemethylamine was thus monitored by ATR-IR spectroscopy and fluorescence microscopy. Similarly, BSA and lysozyme were individually grafted to such Fischer carbene modified surfaces using their pendant lysine residues. It has been demonstrated that the anchored lysozyme retains its bactericidal property.

Graphical abstract: Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM

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Article information

DOI
https://doi.org/10.1039/C0OB00798F
Article type
Paper
Submitted
29 Sep 2010
Accepted
05 May 2011
First published
31 May 2011

Org. Biomol. Chem., 2011,9, 5123-5128

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Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM

P. Dutta, N. Ray, S. Roy, A. Kr. Dasgupta, O. Bouloussa and A. Sarkar, Org. Biomol. Chem., 2011, 9, 5123 DOI: 10.1039/C0OB00798F

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