Issue 12, 2010
From the journal:

Nanoscale

Self-assembled monolayer based impedimetric platform for food borne mycotoxin detection

Pratima Rathee Solanki,a   Ajeet Kaushik,a   T. Manaka,b   Manoj Kumar Pandey,a   M. Iwamoto,b   Ved Varun Agrawala  and  Bansi Dhar Malhotra*ac  

* Corresponding authors

a Department of Science and Technology Centre on Biomolecular Electronics, National Physical Laboratory (Council of Scientific Industrial Research), Dr K. S. Krishnan Marg, New Delhi, India
E-mail: bansi.malhotra@gmail.com
Fax: +91-11-45609310
Tel: +91-11-45609152

b Department of Physical Electronics, Tokyo Institute of Technology, 2-12-1-S3-33, O-Okayama, Maguro, Tokyo, Japan

c Centre for Nano-Bioengineering and Spintronics, Chungnam National University, Daejeon, Korea

Abstract

A self-assembled monolayer (SAM) of 11-amino-1-undecanethiol (AUT) has been fabricated onto a gold (Au) substrate to co-immobilize anti-ochratoxin-A antibodies (AO-IgGs) and bovine serum albumin (BSA) to detect food borne mycotoxin [i.e., ochratoxin-A (OTA)]. AUT/Au electrode, AO-IgGs/AUT/Au immunoelectrode and BSA/IgGs/AUT/Au immunoelectrode have been characterized using scanning electron microscopy (SEM) and electrochemical studies such as cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Electrochemical studies reveal that the AUT-SAM with NH2 groups provide favorable conditions to immobilize AO-IgGs with better orientation, resulting in enhanced electron transport to obtain improved sensing characteristics. The EIS response studies of the BSA/AO-IgGs/AUT/Au immunoelectrode obtained as a function of OTA concentration reveal that the value of the charge transfer resistance (RCT) increases with increased OTA concentration. The BSA/AO-IgGs/AUT/Au immunoelectrode exhibits linearity over 0.5–6.0 ng/dl, detection limit of 0.08 ng/dl using 3σb/m criteria, response time of 30 s and sensitivity of ∼36.83 Ω/ng dl−1 cm−2 with a regression coefficient of 0.999. Attempts have been made to monitor the change in RCT of BSA/AO-IgGs/AUT/Au immunoelectrode on addition of coffee samples.

Graphical abstract: Self-assembled monolayer based impedimetric platform for food borne mycotoxin detection

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Article information

DOI
https://doi.org/10.1039/C0NR00289E
Article type
Paper
Submitted
30 Apr 2010
Accepted
09 Aug 2010
First published
18 Oct 2010

Nanoscale, 2010,2, 2811-2817

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Self-assembled monolayer based impedimetric platform for food borne mycotoxin detection

P. R. Solanki, A. Kaushik, T. Manaka, M. K. Pandey, M. Iwamoto, V. V. Agrawal and B. D. Malhotra, Nanoscale, 2010, 2, 2811 DOI: 10.1039/C0NR00289E

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