Issue 5, 2011
From the journal:

Molecular BioSystems

LIPS arrays for simultaneous detection of antibodies against partial and whole proteomes of HCV, HIV and EBV

Peter D. Burbelo,†*a   Kathleen E. Bren,a   Kathryn H. Ching,a   Emile S. Gogineni,b   Shyam Kottilil,c   Jeffrey I. Cohen,d   Joseph A. Kovacsb  and  Michael J. Iadarolaa  

* Corresponding authors

a Neurobiology and Pain Therapeutics Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, USA
E-mail: burbelop@nidcr.nih.gov
Fax: +1 301-402-0667
Tel: +1 301-402-0778

b Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, USA

c Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA

d Medical Virology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA

Abstract

For many infectious agents, the detection of antibodies is critical for diagnosing, monitoring and understanding vaccine responses. To facilitate the highly quantitative and simultaneous analysis of antibodies against multiple proteins from infectious agents, we have developed Luciferase Immunoprecipitation Systems (LIPS) arrays. By configuring microtiter plates with multiple antigens and testing control and infected serum samples at one time in solution, LIPS arrays provided highly reproducible antibody titers to panels of antigens with a wide dynamic range of detection. While all serum samples showed similar positive and negative immunoreactivity with internal control antigens derived from Influenza and Renillaluciferase-alone protein, respectively, antibody titers to many HCV and HIV antigens were generally 10 to over 400-fold higher in the infected versus uninfected samples. Additional screening of 18 proteins from the EBV proteome with serum samples from healthy EBV-infected individuals showed statistically significant antibody titers to 50% of the proteins tested. Antibody titers for the different EBV antigens in the healthy EBV-infected individuals were markedly heterogeneous highlighting the complexity of host humoral responses. These results suggest that LIPS arrays offer a highly discriminating platform for simultaneously profiling a wide spectrum of antibodies associated with many infectious agents.

Graphical abstract: LIPS arrays for simultaneous detection of antibodies against partial and whole proteomes of HCV, HIV and EBV

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Article information

DOI
https://doi.org/10.1039/C0MB00342E
Article type
Paper
Submitted
19 Dec 2010
Accepted
31 Jan 2011
First published
19 Feb 2011

Mol. BioSyst., 2011,7, 1453-1462

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LIPS arrays for simultaneous detection of antibodies against partial and whole proteomes of HCV, HIV and EBV

P. D. Burbelo, K. E. Bren, K. H. Ching, E. S. Gogineni, S. Kottilil, J. I. Cohen, J. A. Kovacs and M. J. Iadarola, Mol. BioSyst., 2011, 7, 1453 DOI: 10.1039/C0MB00342E

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