Issue 2, 2011
From the journal:

Journal of Materials Chemistry

Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins

Zi-An Lin,*a   Jiang-Nan Zheng,a   Fen Lin,b   Lan Zhang,a   Zongwei Caic  and  Guo-Nan Chen*a  

* Corresponding authors

a Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China
E-mail: gnchen@fzu.edu.cn, zalin@fzu.edu.cn
Fax: +86-591-83713866

b Instrumentation Analysis and Measurement Center of Fuzhou University, Fuzhou, Fujian, China

c Department of Chemistry, Hong Kong Baptist University, Hong Kong, SAR, P. R. China

Abstract

Aminophenylboronic acid (APBA)-functionalized magnetic iron oxide nanoparticles (Fe3O4 MNPs) were synthesized for the selective capture of glycoproteins from unfractionated protein mixtures. The morphology, adsorption, and recognition properties of the resultant particles were investigated and uniform size APBA-coated MNPs with a mean diameter of ∼15 nm and high magnetic saturation value of 30.6 emu g−1 were obtained, which endued the adsorbent with a large surface area and convenience of isolation. The selectivity and binding capacity of APBA-coated MNPs were evaluated by using standard glycoproteins (cellulose and ovalbumin) and nonglycoproteins (bovine hemoglobin, bovine serum albumin and lysozyme) as model samples. Adsorption experiments and SDS-PAGE demonstrated that the APBA-coated MNPs had higher binding capacity and selectivity for glycoproteins compared to nonglycoproteins. In addition, the practicability of the as-prepared MNPs was further assessed by specific capture of ovalbumin from an egg white sample.

Graphical abstract: Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins

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Article information

DOI
https://doi.org/10.1039/C0JM02300K
Article type
Paper
Submitted
16 Jul 2010
Accepted
08 Sep 2010
First published
27 Oct 2010

J. Mater. Chem., 2011,21, 518-524

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Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins

Z. Lin, J. Zheng, F. Lin, L. Zhang, Z. Cai and G. Chen, J. Mater. Chem., 2011, 21, 518 DOI: 10.1039/C0JM02300K

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