Site-specific pKa determination of the carboxylate-binding subunit in artificial peptide receptors

Stephan Niebling; Sunil K. Srivastava; Christoph Herrmann; Peter R. Wich; Carsten Schmuck; Sebastian Schlücker

doi:10.1039/B926514G

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Site-specific pK_a determination of the carboxylate-binding subunit in artificial peptide receptors†

Stephan Niebling,^a Sunil K. Srivastava,^a Christoph Herrmann,^a Peter R. Wich,^b Carsten Schmuck^b and Sebastian Schlücker*^a

Author affiliations

* Corresponding authors

^a University of Osnabrück, Department of Physics, Barbarastr. 7, 49069 Osnabrück, Germany
E-mail: sebastian.schluecker@uos.de
Fax: +49-541-96913592
Tel: +49-541-9693592

^b Institute of Organic Chemistry, University Duisburg-Essen, Universitätsstr. 7, 45117 Essen, Germany
E-mail: carsten.schmuck@uni-due.de
Fax: +49-201-1834259
Tel: +49-201-1833097

Abstract

Site-specific pK_a determination of the carboxylate-binding subunit in artificial peptide receptors is achieved by pH-dependent UV resonance Raman scattering with subsequent matrix factorization. This quantitative methodology allows to determine accurately the local acidity of a binding site based on the complete vibrational spectrum and, most importantly, without the need of selecting any particular peak.

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Supplementary files

1. pH titration reference data; 2. UV RR/NMF data for the small model system CBS-NH ₂; 3. Details on the NMF procedure and the Henderson–Hasselbalch fit PDF (383K)

Article information

DOI: https://doi.org/10.1039/B926514G
Article type: Communication
Submitted: 15 Dec 2009
Accepted: 01 Feb 2010
First published: 17 Feb 2010

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Chem. Commun., 2010,46, 2133-2135

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Site-specific pK_a determination of the carboxylate-binding subunit in artificial peptide receptors

S. Niebling, S. K. Srivastava, C. Herrmann, P. R. Wich, C. Schmuck and S. Schlücker, Chem. Commun., 2010, 46, 2133 DOI: 10.1039/B926514G

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