Issue 5, 2010
From the journal:

Analyst

Fluorescence turn-on detection ofprotamine based on aggregation-induced emission enhancement characteristics of 4-(6′-carboxyl)hexyloxysalicylaldehyde azine†

Xiao-tong Chen,a   Yu Xiang,‡a   Na Li,a   Pan-Shu Songa  and  Ai-jun Tong*a  

* Corresponding authors

a Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, PR China
E-mail: tongaj@mail.tsinghua.edu.cn
Fax: +86-10-62782485
Tel: +86-10-62787682

Abstract

A novel fluorescence turn-on method for sensitive and selective detection of protamine was developed based on aggregation-induced emission enhancement (AIEE) characteristics of 4-(6′-carboxyl)hexyloxysalicylaldehyde azine (CHSA) induced by electrostatic interaction between protamine and CHSA−. Under optimal conditions described, a large Stokes shift of ∼198 nm could be observed, and the fluorescence enhancement at 538 nm was linearly related to the concentration of protamine in the range of 0.6∼18 μg mL−1 with the relative correlation coefficient of R2 = 0.9996 (n = 11) and a detection limit as low as 43 ng mL−1. The relative standard deviation (R.S.D.) was 2.0% (n = 5). The proposed method was successfully utilized in quantifying protamine in diluted horse serum. In addition, due to the special electrostatic association between protamine and heparin, CHSA could also be employed as a probe to study the interaction between them.

Graphical abstract: Fluorescence turn-on detection of protamine based on aggregation-induced emission enhancement characteristics of 4-(6′-carboxyl)hexyloxysalicylaldehyde azine

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Article information

DOI
https://doi.org/10.1039/B925508G
Article type
Paper
Submitted
08 Dec 2009
Accepted
04 Feb 2010
First published
26 Feb 2010

Analyst, 2010,135, 1098-1105

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Fluorescence turn-on detection of protamine based on aggregation-induced emission enhancement characteristics of 4-(6′-carboxyl)hexyloxysalicylaldehyde azine

X. Chen, Y. Xiang, N. Li, P. Song and A. Tong, Analyst, 2010, 135, 1098 DOI: 10.1039/B925508G

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