Issue 6, 2010
From the journal:

Molecular BioSystems

Three-dimensional model of the honeybee venom allergen Api m 7: structural and functional insights

Dessislava Georgieva,a   Kerstin Greunkeb  and  Christian Betzel*a  

* Corresponding authors

a Institute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestrasse 85, Building22a, 22603 Hamburg, Germany
E-mail: Christian.Betzel@uni-hamburg.de
Fax: +49 40 8998 4747
Tel: +49 40 8998 4744

b Protein Ligand Structural (PLS)-Design GmbH, Eichenstrasse 42, Hamburg, Germany

Abstract

Api m 7 is one of the major protease allergens of the honeybee venom. It consists of a serine protease-like (SPL) and a CUB domain. The knowledge about the structure and function of Api m 7 is limited mainly to its amino acid sequence. Three-dimensional models of the two structural domains were constructed using their amino acid sequences and the crystallographic coordinates of prophenoloxidase-activating factor (PPAF-II) as a template for the SPL domain and the coordinates of porcine spermadhesin PSP-II for the CUB domain. The structural organization of Api m 7 suggests that the CUB domain is involved in interactions with natural substrates while the SPL domain probably activates zymogens. IgE epitopes and antigenic sites were predicted. Api m 7 shows structural and functional similarity to the members of the PPAF-II family. Possible substrates, function and evolution of the enzyme are discussed in the paper.

Graphical abstract: Three-dimensional model of the honeybee venom allergen Api m 7: structural and functional insights

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Article information

DOI
https://doi.org/10.1039/B923127G
Article type
Paper
Submitted
04 Nov 2009
Accepted
08 Feb 2010
First published
17 Mar 2010

Mol. BioSyst., 2010,6, 1056-1060

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Three-dimensional model of the honeybee venom allergen Api m 7: structural and functional insights

D. Georgieva, K. Greunke and C. Betzel, Mol. BioSyst., 2010, 6, 1056 DOI: 10.1039/B923127G

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