Issue 3, 2010
From the journal:

Lab on a Chip

Generation of oxygen gradients with arbitrary shapes in a microfluidic device†

Micha Adler,a   Mark Polinkovsky,a   Edgar Gutierreza  and  Alex Groisman*a  

* Corresponding authors

a Department of Physics, University of California, San Diego, 9500 Gilman Drive, MC 0374, La Jolla, CA, USA
E-mail: agroisman@ucsd.edu

Abstract

We present a system consisting of a microfluidic device made of gas-permeable polydimethylsiloxane (PDMS) with two layers of microchannels and a computer-controlled multi-channel gas mixer. Concentrations of oxygen in the liquid-filled flow channels of the device are imposed by flowing gas mixtures with desired oxygen concentrations through gas channels directly above the flow channels. Oxygen gradients with different linear, exponential, and non-monotonic shapes are generated in the same liquid-filled microchannel and reconfigured in real time. The system can be used to study directed migration of cells and the development of cell and tissue cultures under gradients of oxygen.

Graphical abstract: Generation of oxygen gradients with arbitrary shapes in a microfluidic device

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Article information

DOI
https://doi.org/10.1039/B920401F
Article type
Technical Note
Submitted
01 Oct 2009
Accepted
02 Nov 2009
First published
27 Nov 2009

Lab Chip, 2010,10, 388-391

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Generation of oxygen gradients with arbitrary shapes in a microfluidic device

M. Adler, M. Polinkovsky, E. Gutierrez and A. Groisman, Lab Chip, 2010, 10, 388 DOI: 10.1039/B920401F

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