A pH-driven DNA switch based on the A+·G mispair

Jennifer A. Lee; Maria C. DeRosa

doi:10.1039/B918725A

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A pH-driven DNA switch based on the A⁺·G mispair†

Jennifer A. Lee^a and Maria C. DeRosa*^a

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* Corresponding authors

^a Chemistry Department, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada
E-mail: maria_derosa@carleton.ca
Fax: (613) 520-3749
Tel: (613) 520-2600 ext. 3844

Abstract

A new approach to the development of pH-driven DNA switches is presented. Here, the improved stability of the A·G mispair under slightly acidic conditions is exploited. The temperature tunability and the potential generality of the approach are discussed.

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Supplementary files

Detailed experimental procedures, fluorescence spectra ofcontrols, representative melting temperature data and a control pHswitching experiment for D1AT PDF (291K)

Article information

DOI: https://doi.org/10.1039/B918725A
Article type: Communication
Submitted: 09 Sep 2009
Accepted: 05 Nov 2009
First published: 25 Nov 2009

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Chem. Commun., 2010,46, 418-420

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A pH-driven DNA switch based on the A⁺·G mispair

J. A. Lee and M. C. DeRosa, Chem. Commun., 2010, 46, 418 DOI: 10.1039/B918725A

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