Issue 1, 2010
From the journal:

Organic & Biomolecular Chemistry

A novel bimodal lipidic contrast agent for cellular labelling and tumour MRI†

Nazila Kamaly,ab   Tammy Kalber,b   Gavin Kenny,b   Jimmy Bell,b   Michael Jorgensen‡*a  and  Andrew Miller*a  

* Corresponding authors

a Imperial College Genetic Therapies Centre, Department of Chemistry, Flowers Building, Armstrong Road, Imperial College London, London, UK
E-mail: a.miller@imperial.ac.uk
Fax: +44 (0)20 7594 5803
Tel: +44 (0)20 7594 5869

b Metabolic and Molecular Imaging Group, Imaging Sciences Department, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, London, UK

Abstract

We have synthesized a bimodal lipidic molecule bearing both fluorophore and contrast agent signatures on the same structure in order to create a robust bimodal liposome for both magnetic resonance imaging (MRI) and fluorescence microscopy utility. The dual-modality concept considered in the synthesis of this new paramagnetic and fluorescent lipid is valuable in that anatomical information (MRI) as well as very sensitive localization (ex vivo fluorescence microscopy) of signal, and therefore liposome biodistribution, is obtainable. Bimodal cationic and neutral PEGylated liposomes were formulated using this novel lipid probe and used to label cells in vitro and image human ovarian xenografts in vivo. Tumour signal enhancement was increased by over 6-fold post-administration of the neutral PEGylated liposomes, and was maintained at this level up to the 24 h end-point. Our results showed this lipid to be more effective and sensitive than the single signature paramagnetic lipid Gd·DOTA·DSA at cellular labelling and tumour MRI.

Graphical abstract: A novel bimodal lipidic contrast agent for cellular labelling and tumour MRI

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Article information

DOI
https://doi.org/10.1039/B910561A
Article type
Paper
Submitted
29 May 2009
Accepted
29 Sep 2009
First published
05 Nov 2009

Org. Biomol. Chem., 2010,8, 201-211

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A novel bimodal lipidic contrast agent for cellular labelling and tumour MRI

N. Kamaly, T. Kalber, G. Kenny, J. Bell, M. Jorgensen and A. Miller, Org. Biomol. Chem., 2010, 8, 201 DOI: 10.1039/B910561A

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