Issue 6, 2009
From the journal:

Chemical Communications

High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray†

Hongyan Sun,a   Lay Pheng Tan,b   Liqian Gaoac  and  Shao Q. Yao*abc  

* Corresponding authors

a Department of Chemistry, National University of Singapore, 3 Science Drive, Singapore 11754
E-mail: chmyaosq@nus.edu.sg
Fax: +65 (0)6779169
Tel: +65 (0)65162925

b Department of Biological Sciences, National University of Singapore, 3 Science Drive, Singapore 11754

c NUS MedChem Program of the Office of Life Sciences, National University of Singapore, 3 Science Drive, Singapore 11754

Abstract

We report herein a novel phosphopeptide microarray capable of noncovalently “trapping” catalytically inactive mutants of protein tyrosine phosphatases (PTPs), and its application in high-throughput determination of PTP substrate specificity.

Graphical abstract: High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray

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Article information

DOI
https://doi.org/10.1039/B817853D
Article type
Communication
Submitted
10 Oct 2008
Accepted
26 Nov 2008
First published
12 Dec 2008

Chem. Commun., 2009, 677-679

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High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray

H. Sun, L. P. Tan, L. Gao and S. Q. Yao, Chem. Commun., 2009, 677 DOI: 10.1039/B817853D

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