Issue 11, 2008
From the journal:

Journal of Analytical Atomic Spectrometry

Quantification of selenium-tagged proteins in human plasma using species-unspecific isotope dilution ICP-DRC-qMS coupled on-line with anion exchange chromatography†

Ming Xu,a   Limin Yanga  and  Qiuquan Wang*ab  

* Corresponding authors

a Department of Chemistry & the MOE Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
E-mail: qqwang@xmu.edu.cn
Fax: +86 5922181796
Tel: +86 5922181796

b State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China

Abstract

Inductively coupled plasma dynamic reaction cell-quadrupole mass spectrometry (ICP-DRC-qMS) coupled on-line with anion exchange chromatography (AEC) has been developed for the quantification of selenium-tagged proteins in human plasma. Methane was employed as a reaction gas in the dynamic reaction cell to achieve the determination of 80Se free of spectroscopic interference from 40Ar2+ and 79BrH+. Five selenium species including selenoprotein P (SelP), glutathione peroxidase (GPx), selenoalbumin (SeAlb), and two unknown selenospecies (U1 and U2) in a pooled plasma sample from five healthy people were separated using AEC, and the distribution of selenium in SelP, GPx, SeAlb, U1 and U2 (about 45.5%, 19.1%, 15.1%, 2.9% and 8.1%) was determined by ICP-DRC-qMS using species-unspecific isotope dilution (80Se/77Se). Based on the detection limit (DL) of selenium (0.54 ng mL−1), we estimated that the DLs for SelP and GPx were 0.59 pmol mL−1 and 1.7 pmol mL−1, respectively. Through the stoichiometry of the selenium atom in the selenium-tagged proteins, SelP (2.7 ± 0.1 μg mL−1) and GPx (5.4 ± 0.2 μg mL−1) were successfully quantified.

Graphical abstract: Quantification of selenium-tagged proteins in human plasma using species-unspecific isotope dilution ICP-DRC-qMS coupled on-line with anion exchange chromatography

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Supplementary files

Article information

DOI
https://doi.org/10.1039/B804935A
Article type
Technical Note
Submitted
26 Mar 2008
Accepted
14 Jul 2008
First published
28 Aug 2008

J. Anal. At. Spectrom., 2008,23, 1545-1549

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Quantification of selenium-tagged proteins in human plasma using species-unspecific isotope dilution ICP-DRC-qMS coupled on-line with anion exchange chromatography

M. Xu, L. Yang and Q. Wang, J. Anal. At. Spectrom., 2008, 23, 1545 DOI: 10.1039/B804935A

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